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KMID : 0811720020060000155
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Korean Journal of Physiology & Pharmacology
2002 Volume.6 No. 0 p.155 ~ p.0
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Biological Actions of Dendroaspis Natriuretic Peptide in Rabbit Kidney
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Kim Soo-Mi
Kim Sung-Zoo
Cho Kyung-Woo
Kim Sun-Hee
Park Sung-Hun
Kim Yoon-Ah
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Abstract
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Dendroaspis natriuretic peptide (DNP), as new member of natriuretic peptide family, shared functionally important sequence homologies with ANP, BNP, and CNP. Although DNP was firstly identified in reptilian species, synthetic DNP could exert vasorelaxations of rat aorta and canine coronary artery with a similar potency of mammalian ANP. Moreover, production of cGMP via activation of particulate guanylyl cyclase (GC) was increased in a dose-dependent manner by DNP in mammalian aortic endothelial cells. Therefore, the purpose of the present study is to elucidate the physiological role of DNP on renal function and its cellular mechanism in normal animals. Unilateral renal arterial infusion of DNP on renal function was investigated in unanesthetized normal rabbits. DNP infused into left renal artery of rabbit caused increases in urine flow and urinary excretion of chloride. These renal actions induced by DNP were more effective than those by ANP. We have performed experiment to characterize cGMP production by DNP, to compare the quantitative and qualitative differences of this cGMP production by DNP and other natriuretic peptides, and to clarify the relationship between DNP specific receptor and other membrane-bound proteins for activation of particulate GC. Production of cGMP by DNP via activation of particulate GC in membrane proteins obtained from various renal structures was stimulated with a rank order of glomerulus＞outer medullary tubules＞inner medullary tubules. And these were stimulated by various natriuretic peptides with a rank order of potency of ANP＞DNP＞BNP＞＞CNP. For activation of specific receptor by DNP, protein kinase C and adenylyl cyclase could be inhibited cGMP production, and there was not affected by G protein, tyrosine kinase and phosphatase. Our observations indicate that synthetic DNP may have a role as renal regulating peptide via specific receptor with GC domain. (Supported by KOSEF Grant R05-2000-000-00193-0)
Source: Korean Journal of Physiology & Pharmacology.2002 Oct;6(Suppl II):S107-S107
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KEYWORD
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DNP, receptor, cGMP, kidney
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